Journal: Communications Biology

Article Title: tRF16 affects NFKBIA stability and promotes osteoarthritis progression by regulating ALKBH5 expression in m6A-dependent manner

doi: 10.1038/s42003-025-08299-y

Figure Lengend Snippet: A , B Volcano and heat maps of smallRNAs differentially expressed in cartilage tissues of three normal subjects and three OA patients analyzed using Arraystar Human SmallRNA Expression Microarray; C flow diagram of a rat OA model established by meniscectomy. The illustration is created by Biorender.com; D microCT confirms arthritic symptoms in rats at 16 weeks postoperatively; E the Tb.N, Tb.sp and BV/TV value according to the micro-CT; F – H , HE, PAS, and SAFG staining to detect pathological structural alterations in the cartilage tissues of rat knee joints; I RT-qPCR to detect tRF16 expression in the cartilage tissues of rats at 0, 4, 8, 12, and 16 weeks postoperatively; J RT-qPCR to detect the mRNA levels of Aggrecan, COL2A1, MMP1, and MMP13 in the cartilage tissues of rats; K the correlation between tRF16 expression in the knee joints of OA rats at week 16 and the Mankin score. Each group contained 6-8 rats; the data were presented as dot and whsikers and statistically analyzed using Student t -test or 2-way ANOVA, followed by Tukey’s multiple comparison test for post hoc tests, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In the present study, we used Arraystar Human SmallRNA Expression Microarray to analyze the differentially expressed small RNA in cartilage tissue and observed significantly overexpressed tRF16 in OA patients.

Techniques: Expressing, Microarray, Micro-CT, Staining, Quantitative RT-PCR, Comparison

Journal: Toxicological Sciences

Article Title: Chronic Hexavalent Chromium Exposure Upregulates the RNA Methyltransferase METTL3 Expression to Promote Cell Transformation, Cancer Stem Cell-Like Property, and Tumorigenesis

doi: 10.1093/toxsci/kfac023

Figure Lengend Snippet: Total RNA N6-methyladenosine (m6A) modification levels are significantly increased in chronic hexavalent chromium [Cr(VI)] exposure-transformed human bronchial epithelial cells and chronic chromate-exposed mouse lung tissues. A, The heatmap from m6A microarray analysis showing the extent of total messenger RNA m6A methylation in passage-matched control cells (BEAS-2B-Control) and chronic Cr(VI) exposure-transformed cells [BEAS-2B-Cr(VI)]. B and C, Relative total RNA m6A levels measured by using the EpiQuik m6A RNA Methylation Quantification Kit. The total RNA m6A levels in Cr(VI)-transformed cells (B) or chromate-exposed mouse lung tissues (C) are expressed relative to the passage-matched control cells (means ± SD, n = 3) (B) or vehicle control-exposed mouse lungs (means ± SD, n = 6) (C), respectively. *p < .05.

Article Snippet: Human m 6 A RNA modification microarray analysis Total RNAs from chronic Cr(VI) exposure-transformed BEAS-2B cells [BEAS-2B-Cr(VI)] and the passage-matched control BEAS-2B cells (BEAS-2B-Control) were submitted to Arraystar Inc. (Rockville, Maryland) for human m 6 A RNA modification microarray analysis.

Techniques: Modification, Transformation Assay, Microarray, Methylation, Control

Journal: Physiological Genomics

Article Title: Circular RNAs in rat models of cardiovascular and renal diseases

doi: 10.1152/physiolgenomics.00064.2017

Figure Lengend Snippet: Box plots show the distribution of expression levels for all samples in the circular (circ)RNA microarray data set. R-1, R-2, and R-3 are 3 individual Dahl salt-resistant rats (R). S-1, S-2, and S-3 are 3 individual Dahl salt-sensitive rats (S). WKY-1, WKY-2, and WKY-3 are 3 individual Wistar Kyoto rats (WKY). SHR-1, SHR-2, and SHR-3 are 3 individual spontaneously hypertensive rats (SHR).

Article Snippet: Arraystar Rat Circular RNA Microarray ( www.arraystar.com ) was used for microarray experiments.

Techniques: Expressing, Microarray

Journal: Physiological Genomics

Article Title: Circular RNAs in rat models of cardiovascular and renal diseases

doi: 10.1152/physiolgenomics.00064.2017

Figure Lengend Snippet: Differentially expressed circRNAs between normotensive and hypertensive rats. The figure represents the number of circRNAs differentially expressed according to the microarray analysis. Based on the location and direction on the genome, circRNAs are subcategorized into exonic, intronic, antisense, sense overlapping, and intergenic. S vs. R up indicates circRNAs were upregulated in Dahl salt-sensitive rats (S) compared with Dahl salt-resistant rats (R). S vs. R down indicates circRNAs were downregulated in S compared with R. SHR vs. WKY up indicates circRNAs were upregulated in spontaneously hypertensive rats (SHR) compared with Wistar Kyoto rats (WKY). SHR vs. WKY down indicates circRNAs were downregulated in SHR compared with WKY.

Article Snippet: Arraystar Rat Circular RNA Microarray ( www.arraystar.com ) was used for microarray experiments.

Techniques: Microarray

Journal: Physiological Genomics

Article Title: Circular RNAs in rat models of cardiovascular and renal diseases

doi: 10.1152/physiolgenomics.00064.2017

Figure Lengend Snippet: Validation of differentially expressed circRNAs in the microarray study by quantitative RT-PCR. R, Dahl salt-resistant rat; S, Dahl salt-sensitive rat; WKY, Wistar Kyoto rat; SHR, spontaneously hypertensive rat. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Arraystar Rat Circular RNA Microarray ( www.arraystar.com ) was used for microarray experiments.

Techniques: Biomarker Discovery, Microarray, Quantitative RT-PCR